25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit

Description

Synonym: 25-Hydroxycholecalciferol, Calcidiol. This 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit applies to the in vitro quantitative determination of Universal 25-HVD3 concentrations in serum, plasma and other biological fluids. Type:Competitive, Assay time:1.5h, Detection type:Colormetric, Precision:CV<10%, Assay Range:3.13-200ng/mL, Sensitivity:1.88ng/mL, Recovery:80%-120%, Cross reactivity:The kit detected 25-HVD3 in the samples and no significant cross-species reactions were found. Detection Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 25-HVD3. During the reaction, 25-HVD3 in the sample or standard competes with a fixed amount of 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.