5-TAMRA Cadaverine

Description

5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators e.g. EDC, or DCC or activated esters e.g. NHS esters through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base AAasAasAa which can be reduced to a generate stable amine derivative by sodium borohydride NaBH4 or sodium cyanoborohydride NaCNH3 . Although the mixed isomers of 5 6 -TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5 6 -TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.