5X One Step PrimeScriptTM III RT-qPCR Kit, ROX plus, GPR

Description

5X One Step PrimeScript III RT-qPCR Kit contains a more concentrated version of the premix in our 2X One Step PrimeScript III RT-qPCR Mix RR600 . For example, only 5 mul of the 5X premix is needed for a 25 mul reaction versus 12.5 mul of 2X premix. This opens possibilities for using a more dilute template or multiple primers and probes. As with the 2X premix, the 5X premix does not freeze at its storage temperature of -20C, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The RT enzyme displays tolerance to elevated temperatures up to 55C . At higher temperatures, RNA secondary structure is more relaxed and less likely to impede extension. So, the elevated temperature tolerance enables cDNA synthesis from RNA with a more complex secondary structure. Cat. # XU0001 and 638336 contain ROX Reference Dye, optimized to be compatible with various qPCR cyclers, in the master mix. Cat. # 638336 contains five of Cat. # XU0001, sufficient to perform 1,000 x 25-mul reactions. Please refer to Cat. # XU0001 for complete product documentation and resources. This product is a general purpose reagent intendedFor Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the users responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information. Back Back Back Back Back Back