5X PrimePathTM Probe qPCR Kit, no ROX, GPR

Description

The 5X PrimePath Probe qPCR Kit, no ROX, GPR is a real-time PCR master mix containing TaKaRa Taq HS premixed with the buffers needed for probe-based qPCR using DNA samples. The 5X concentration of the master mix allows for increased sample volumes to improve sensitivity or the ability to add additional primer/probes for the development of multiplex assays. Cat. # 638347 contains reagents sufficient to perform 1,000 x 25 mul reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Dynamic range of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Human cDNA was amplified using humanGAPDHprimers/probes with 5X PrimePath Probe qPCR Kit, no ROX plus, GPR on a CFX96 Real-Time PCR System. Two replicates of a sixfold dilution series of human cDNA were tested. The amplification plot for the dilution series is shown. Back Lot-to-lot consistency of 5X PrimePath Probe qPCR Kit, no ROX, GPR. Three separate lots of 5X PrimePath Probe qPCR Kit, no ROX, GPR were used to amplify 100 ng, 10 ng, 1 ng, 100 pg, or 10 pg of human cDNA using a human GAPDH primer/probe set. The Cq for each amount of human cDNA using Lot 1, Lot 2, or Lot 3 of 5X PrimePath Probe qPCR Kit, no ROX, GPR are shown Back