AAVpro Concentrator

Description

AAVpro Concentrator provides a simple, highly efficient alternative to ultracentrifugation for concentrating AAV particles of any serotype from cell culture supernatant. In contrast with other concentration methods that involve only precipitation of AAV particles, AAVpro Concentrator incorporates filtration steps to yield AAV preps of higher purity. The scalable mix-and-spin protocol can accommodate a range of input volumes and requires less than one hour of hands-on time. The AAVpro Concentrator reagent is provided as a 4X concentrate, which can be applied in proportion to the amount of culture medium being processed. The kit includes reagents and filtration columns for processing up to 150 ml of culture supernatant for two samples. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back An outline of the AAV vector enrichment process using AAVpro Concentrator. Back Concentration and recovery rate of AAV1 vectors, concentrated using AAVpro Concentrator. In this experiment, culture supernatant containing AAV1 vectors expressing ZsGreen1 was concentrated from a starting input of 40 ml one T225 flask down to a final volume of 200 mul using AAVpro Concentrator, for a 95-fold increase in AAV titer and a recovery rate of 48 %. Back AAV titration in transducing units, as determined by FACS analysis of ZsGreen1 expression in HT1080 cells. The cells were infected with AAV at 5,000 or 500 vg/cell MOI and analyzed by flow cytometry after three days. Concentrated AAV particles administered at MOIs of 5,000 or 500 were able to infect cells at rates of 44.7 % or 9.2 %, respectively. Back