AAVpro CRISPR/SaCas9 Vector System

Description

The AAVpro CRISPR/SaCas9 Vector System is a complete system for cloning a target single guide RNA sgRNA used in CRISPR/Cas9-mediated genome editing in mammalian cells. The pAAV-Guide-it-1 Vector is supplied pre-linearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. It also contains the Cas9 gene fromStaphylococcus aureus SaCas9 expressed from an upstream CMV promoter. SaCas9 has a genome editing efficacy similar to the more commonly usedStreptococcus pyogenesCas9 SpCas9 , but is about 1 kb shorter which allows it to be packaged into a single AAV vector. It is important to note that SaCas9 recognizes a different PAM sequence; please check the user manual for details. This kit also contains all of the components needed for cloning including the ligation mix, necessary controls, and Stellar Competent Cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Back