AAVpro Helper Free System (AAV5-CRE Recombinase)

Description

This system is used to prepare AAV5 particles that will deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence loxPsite containing an 8-bp core sequence where recombination takes place. The orientation and location of introducedloxPsites allow for Cre-mediated gene inversion, translocation, or deletion. With this kit, high-titer AAV5 particles can be prepared without a helper virus by simply transfecting HEK 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV5 particles. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back pRC5 vector map. Back AAVpro Helper Free System workflow.