AAVpro Purification Kit (AAV2)

Description

The purity and titer of the AAV vector is an important for achieving stable and effective introduction of genes into individual animals and cultured cells. Methods such as CsCl concentration gradient ultracentrifugation and iodixanol ultracentrifugation are commonly used to purify AAV2 vectors, but such methods are time-consuming and require careful technique to obtain high yields. The AAVpro Purification Kit AAV2 allows for simple and fast 4 hours AAV2 particle purification from AAV2-producing cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Recovery, purity, and infectivity of purified AAV2 particles .AAV2 particles were purified with each companys purification kit from virus-producing cells. The yield of each purified AAV2 vector was evaluated by real-time PCR Panel A , and the purity of each purified AAV2 vector was evaluated by SDS-PAGE using 1 x 109vg per lane Panel B . Infectivity was measured by infection of HT1080 cells with purified AAV2 particles at 2,500 vg/cell and subsequent measurement of ZsGreen expression Panel C . Back