AAVpro Purification Kit Maxi (All Serotypes)

Description

The AAVpro Purification Kit Maxi All Serotypes is a four-prep kit that can be used for purifying AAV of any serotype without the need for ultracentrifugation. The purification yields highly pure AAV particles that can be used directly forin vivotransduction or transduction of cultured cells. Cryonase Cold-Active Nuclease is included. This product allows for purification of four virus preps, each from a culture size of 5 x T225 flasks. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Summary of the AAV particle purification protocol using the AAVpro Purification Kit Maxi All Serotypes . Back Purity of viral particles collected using the AAVpro Purification Kit Maxi All Serotypes .AAV viral particles of each serotype carrying the fluorescent protein ZsGreen1 were prepared from producer cells cultured in five T225 flasks. The AAV solution was purified using this product, and viral titer was measured using the AAVpro Titration Kit for Real Time PCR Ver. 2. SDS-PAGE was performed on each sample, with 1 x 109vector genomes loaded per lane. For all serotypes, the AAV capsid proteins VP1, VP2, and VP3 were observed to be the major bands present, confirming the purity of the AAV viral particles. Back Infection capacity of AAV particles purified with the AAVpro Purification Kit Maxi All Serotypes .Infectivities of AAV particles of various serotypes were evaluated. Purified AAV particles carrying the fluorescent protein ZsGreen1 were used for infection at 5,000 vector genomes per cell serotypes 1, 2, 3, and 6 or 50,000 vector genomes per cell serotype 5 . Flow cytometry was performed three days later. Results indicate the AAV particles purified using this kit were able to infect the cell lines tested. Back