AAVpro Purification Kit Midi (All Serotypes)

Description

The AAVpro Purification Kit Midi All Serotypes is a four-prep kit that can be used for purifying AAV of any serotype without the need for ultracentrifugation. The purification yields highly pure AAV particles that can be used directly forin vivotransduction or transduction of cultured cells. Cryonase Cold-Active Nuclease is included. This product allows for purification of four virus preps, each from a culture size of 1 x T225 flask. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Gene transduction of iPSC-derived cardiomyocytes using AAV1, 2, 5, and 6.AAV1, 2, 5, and 6 vectors carrying theZsGreen1gene were purified with the AAVpro Purification Kit Midi All Serotypes kit. Each purified vector was used for viral transduction of iPSC-derived cardiomyocytes at 500,000 vector genomes per cell. After 72 hr of infection, ZsGreen1 fluorescence was analyzed by microscopy, with an exposure time of 0.2 sec. AAV5 was additionally exposed at 0.5 sec. NC = negative control. Back