ACTB CRISPR Stable HEK293T Cell Line

Description

beta-Actin ACTB was integrated into a specific genomic loci using CRISPR/Cas9 in HEK293T cells. The transgene is driven by endogenous promoters to ensure consistent and predictable expression of ACTB. A protein quantification reporter PQR was incorporated between the endogenous and RPL13A genes. The PQR allows equimolar production of the endogenous protein, the recombinant protein, and a fluorescent reporter. As a result, expression levels of both the endogenous and recombinant proteins can be continuously monitored using fluorescence.