Adeno-XTM 293 Cell Line

Description

The Adeno-X 293 Cell Line is a low-passage transformed human embryonic kidney cell line for the production of adenovirus stocks. Adenovirus stocks can be produced by either transfecting this cell line with a linearized adenoviral vector such as the linear vectors supplied with any Adeno-X System 3 or infecting with a viable adenovirus seed stock. The slower growth rate exhibited by this line reduces cell death due to overconfluence, and therefore facilitates more efficient rescue and amplification. These cells also possess strong adherence and are highly transfectable. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Constructing recombinant adenovirus with In-Fusion Cloning technology.DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed intoE. coli Stellar Competent Cells and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue. Back Back During adenovirus amplification and rescue, Adeno-X 293 cells typically remain intact but round up and may detach from the plate.These changes are collectively referred to as the cytopathic effect CPE . These images show CPE following transfection of Adeno-XZsGreen1DNA into the Adeno-X 293 Cell Line.