Adeno-XTM Adenoviral System 3 (CMV, Red)

Description

Adeno-X Adenoviral System 3 CMV, Red provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-DsRedExpress included in the vector set is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology. Simply PCR-amplify your gene and combine it with pAdenoX-DsRedExpress in an In-Fusion HD Cloning reaction. In-Fusion HD Cloning is fast, simple, precise and efficient, making Adeno-X Adenoviral System 3 the most advanced commercially available adenoviral gene delivery tool. Adenoviral constructs created with this system will constitutively express your protein of interest and the fluorescent protein DsRed-Express for visualization of infected mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Constructing recombinant adenovirus with In-Fusion Cloning technology.DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed intoE. coli Stellar Competent Cells and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue. Back Adeno-X adenovirus expressing DsRed-Express delivered to primary neural cells.Cells were infected overnight at 37C, washed, and incubated in complete medium. After 48 hr, cells were visualized using fluorescence microscopy. Back