Adeno-XTM Maxi Purification Kit

Description

A complete filtration-based purification system for concentrating and purifying infectious adenovirus. The Adeno-X Maxi Purification Kit is an alternative to CsCl density gradient ultracentrifugation. Each kit provides the buffers and filters needed to purify adenovirus from cell lysate. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Overview of the Adeno-X Maxi Purification Kit protocol. Back The filtration apparatus of the Adeno-X adenovirus purification kits are preassembled and includes a one-way valve to simplify the purification process.The one-way valve eliminates the need for repeated syringe removals when binding and washing. The adenoviral stock is brought into the syringe through the valve and pushed out through the purification filter cartridge by the syringe plunger. Back Back Adenovirus purification with high yield.HEK-293 cells were seeded and infected with adenovirus and harvested when the cytopathic effect was nearly complete. The resulting adenovirus was then purified according to the Adeno-X Maxi Purification Kit protocol and total IFU was determined for each fraction crude, flowthrough, wash, and eluate . 72 % of the starting material was recovered in the eluate as purified virus. The process was efficient and fast, allowing us to purify 4.68 x 1010adenoviral particles in one hour. Back