Alcian Blue Staining Kit,pH 0.4

Description

EnkiLife provide Alcian Blue Staining Kit,pH 0.4. Alcian Blue, also known as Alcian Blue 8GX or Ingrain Blue 1, is a copper-containing phthalocyanin dye originally used in textile dyeing. This cationic dye binds to acidic groups; specifically, Alcian Blue forms insoluble complexes with anionic groups such as carboxyl and sulfate residues in tissues. The dye molecule consists of a central copper-containing phthalocyanine ring linked via thioether bonds to four isothiourea groups that confer a moderate basicity and a net cationic charge. At pH 2.5, carboxyl groups in tissues are ionized and carry a negative charge, forming electrostatic (salt) linkages with the cationic dye; consequently, acidic mucosubstances rich in carboxyl groups (sulfated and sialylated mucins) are stained. At pH < 1.0, sulfate groups are ionized and negatively charged, allowing Alcian Blue to bind strongly to sulfate-rich mucosubstances. Neutral mucins (e.g., those in gastric mucosa and Brunner’s glands) do not react with Alcian Blue under any of these conditions.EnkiLife Alcian Blue Staining Solution (pH 0.4) exploits these pH-dependent binding properties to discriminate mucosubstance classes: pH 2.5: carboxyl (-COOH) groups stain strongly; sulfate-containing mucins stain weakly. pH 1.0: carboxyl groups are unstained; sulfate-containing mucins stain. pH 0.4: only strongly acidic sulfated mucosubstances are stained.Application: Alcian Blue stain