AMY3 Stable HEK293 Cell Line

Description

The AMY3-HEK293 stable cell line was created by co-transfecting pcDNA3-CTR with pBud-GFP-RAMP3 at a 1:1 molar ratio, enabling reliable expression of the Amylin receptor-3 AMY3 complex. AMY3 is a heterodimeric receptor formed by calcitonin receptor CTR and receptor activity-modifying proteins RAMPs , belonging to the class B GPCR family. It has been implicated in the downstream signaling of both amyloid-beta Abeta and human amylin hAmylin , with receptor activation shown to increase intracellular cAMP through Gs proteinmediated stimulation of adenylate cyclase. This stable cell line provides an ideal model for investigating the signaling pathways triggered by Abeta and hAmylin, and for exploring whether their cytotoxic effects are specifically mediated through the AMY3 isoform of the amylin receptor.