ANTIBIOTIC ASSAY MEDIUM NO.1 (SEED AGAR) (VEG.)

Description

Antibiotic Veg Assay Medium No.1 (Seed Veg Agar) is prepared by replacing animal based peptones with vegetable peptones, making the medium BSE-TSE risks free. It can be used for the same purpose of Antibiotic Assay Medium No.1 (Seed Agar). The potency of an antibiotic can be determined by chemical, physical and biological assays. Biological assays offer the most convenient method, since a reduction in the antimicrobial activity of a specific antibiotic is not usually displayed in chemical methods. Biological testing may be performed by either dilution (turbidimetric) or diffusion methods. The choice of methodology is often based on many factors, including relative ease of performance, flexibility and use of automated or semi-automated devices for both identification and susceptibility testing. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays. Antibiotic Veg Assay Medium No.1 is used in the microbiological assay of ?-lactam and other antibiotics and as seed agar with Micrococcus luteus (ATCC 9341) for plate assay of Bacitracin, with Staphylococcus aureus (ATCC 29739) for cylinder plate assay of Cephalexin, Cephalothin, Cephapirin, Cloxacillin, Dicloxacillin, Methicillin, Nafcillin , Oxacillin, Penicillin-G and Staphylococcus epidermidis (ATCC 12228) for plate assay of Novobiocin. This media can be used according to the specifications detailed in various pharmacopoeias.