ATG4A/B Stable Double Knockout HeLa Cell Line

Description

The ATG4A/B Stable Double Knockout HeLa Cell Line is engineered to lack both ATG4A and ATG4B genes, critical enzymes in the autophagic process. Through the use of CRISPR-Cas9, this cell line demonstrates a compounded impairment in autophagy, with drastically reduced processing of both LC3 and GABA type A receptor-associated protein-like 1 GABARAPL1 , although some residual autophagic activity remains due to the presence of ATG4C and ATG4D. The dual knockout nature of this cell line makes it a powerful model for studying the functional redundancy among ATG4 isoforms and the specific contributions of ATG4A and ATG4B to cellular homeostasis, autophagosome formation, and protein turnover. Complete list of cell lines from this panel: Cat. T3588 - ATG4B Stable Knockout HeLa Cell Line Cat. T3589 - ATG4A/B Stable Double Knockout HeLa Cell Line Cat. T3590 - ATG4A/B/C Stable Triple Knockout HeLa Cell Line