ATG4B Stable Knockout HeLa Cell Line

Description

The ATG4B Stable Knockout HeLa Cell Line was developed using CRISPR-Cas9 technology to specifically disrupt the ATG4B gene, a key cysteine protease involved in autophagy, particularly in the lipidation and processing of LC3 and GABA type A receptor-associated protein GABARAP family proteins. This cell line exhibits a severe but incomplete defect in autophagy, characterized by an impaired ability to lipidate LC3B, resulting in a significant reduction in autophagosome formation and cargo degradation. However, residual lipidation of GABARAPL1 and GABARAPL2 can still occur due to the compensatory activity of other ATG4 isoforms. This unique cell line is invaluable for research focused on dissecting the specific roles of ATG4B in autophagy and its contribution to cellular processes such as protein aggregation, intracellular trafficking, and degradation. Complete list of cell lines from this panel: Cat. T3588 - ATG4B Stable Knockout HeLa Cell Line Cat. T3589 - ATG4A/B Stable Double Knockout HeLa Cell Line Cat. T3590 - ATG4A/B/C Stable Triple Knockout HeLa Cell Line