AXL Stable Ba/F3 Cell Line

Description

Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The AXL Stable Ba/F3 Cell Line expresses activated human AXL tyrosine kinase that is linked to a GFP reporter GFP-ETV6-AXL in SED2 vector . Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3. Due to the fusion, kinase became autophosphorylated resulting in activated proliferation and survival pathways in the cells to be independent on IL-3. This cell line carries the SED2 vector which does not have any selection markers.