B. Subtilis Secretory Protein Expression System

Description

Bacillus subtilishas become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe GRAS , its no wonder that the majority of industrially-produced enzymes are expressed inBacillusspecies such asB. subtilis. Optimization of secretion, however, can be necessary to achieve highest yields. To address this, theB. subtilisSecretory Protein Expression System from Takara Bio allows rapid development of a library ofB. subtilisclones, each bearing a pBE-S construct in which the ORF for your protein of interest is fused with sequences for 173 unique signal peptides. Perform a downstream assay to identify and select clones which secrete the highest amount of functional protein into the culture media, and you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein. NOTE:For commercial use of this product, please refer to theprotein expression vectors for commercial use pagefor ordering information. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back