BacPAKTM Baculovirus Expression System

Description

Complete kit for expressing recombinant proteins at high levels. The BacPAK System uses the baculovirusAutographa californicanuclear polyhedrosis virus AcMNPV to produce target proteins in insect cells. More than 90 % of the viruses produced by the transfected cells carry the target protein. The expressed recombinant protein is similar in structure, biological activity, and immunological reactivity to the naturally occurring protein because insect host cells provide post-translational processing similar to that of mammalian cells. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Back Required Products IPLB-Sf21 insect cells may be used as a host for propagating theAutographa californicamultiple-enveloped nuclear polyhedrosis virus AcMNPV and its expression vector derivatives generated from ourBacPAKsystem. The IPLB-Sf21 cell line isderived from pupal ovaries of the fall armyworm,Spodoptera frugiperda. Exponentially growing IPLB-Sf21 cells are concentrated by centrifugation and frozen in insect cell complete medium containing 10 % dimethysulfoxide DMSO . Back Back Back