BcaBESTTM Isothermal FluorDetect Kit (DNA/RNA)

Description

TheBcaBEST Isothermal FluorDetect Kit DNA/RNA allows for rapid detection of DNA or RNA targets via LAMP or one-step RT-LAMP. The kit includesBcaBEST Isothermal Mix 2X and TB Green Solution 300X . Cat. # RR385A contains enough reagents for 100 reactions. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back BcaBEST Isothermal FluorDetect Kit DNA/RNA achieved rapid detection of DNA and RNA samples.BcaBEST Isothermal FluorDetect Kit DNA/RNA was used to amplify DNA and RNA samples template amount = 1, 10, 100, or 1,000 pg or 5, 50, 500, or 5,000 copies at n = 2. The time to reach a constant fluorescence intensity was measured. For these samples, detection was generally achieved within 20 min. The 1/2 in the figure indicates that one of the n = 2 was not detected within 30 min. For the NTC no-template control , no amplification within 30 min was observed except for one NTC fororf-134. RNA samples: HL-60 total RNA ACTBandALAS1 and SARS-CoV-2 RNA controls Twist Bioscience . DNA samples:E. coligenomic DNA fecA and lambda phage genomic DNA orf-134 . Back BcaBEST Isothermal FluorDetect Kit DNA/RNA has a faster detection time compared to other companies reagents using total RNA input.BcaBEST Isothermal FluorDetect Kit DNA/RNA and fluorescent LAMP detection reagents from various companies were used to amplify HL-60 total RNA ACTB; template amount 1, 10, 100, or 1,000 pg at n = 2. The time to reach a certain fluorescence intensity was measured. TheBcaBEST Isothermal FluorDetect Kit DNA/RNA was capable of detection within 15 min. Back BcaBEST Isothermal FluorDetect Kit DNA/RNA has a slightly faster detection time compared to other companies reagents using nasopharyngeal fluid and urine inputs.BcaBEST Isothermal FluorDetect Kit DNA/RNA and fluorescent LAMP detection reagents from various companies were used to detect SARS CoV-2 noninfectious; 1,000 copies/rxn spiked into nasopharyngeal fluid amount = 0, 0.1, or 1 mul/rxn at n = 2,Chlamydia trachomatis noninfectious; 180 copies/rxn spiked into urine amount = 0, 2.25, or 4.5 mul/rxn at n = 3, andNeisseria gonorrhoeae noninfectious; 180 copies/rxn spiked into urine amount = 0, 2.25, or 4.5 mul/rxn at n = 3. The spiked samples were treated with Proteinase K and then heat inactivated before the assay. The time to reach a certain fluorescence intensity was measured. Back BcaBEST Isothermal FluorDetect Kit DNA/RNA is compatible with Q-Probe detection.BcaBEST Isothermal FluorDetect Kit DNA/RNA was used to amplifyChlamydia trachomatiscontrol DNA template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6 andNeisseria gonorrhoeaecontrol DNA template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6 . The time for the fluorescence of TB Green intercalator or Q-Probe to reach the threshold were measured. Q-Probe hybridizes with amplified LAMP products, indicating decreasing fluorescence. The Q-Probe exhibits the same detection time as the intercalator but demonstrates more specificity in this assay.