BD CytopermTM Permeabilization Buffer Plus

Description

Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system. Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system. Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system. Show More Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system. Show More Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system. Flow cytometric analysis of DNA synthesis by TK-1 cells. TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using BD Cytoperm Permeabilization Buffer Plus in the procedure from the BD Pharmingen FITC and APC BrdU Flow Kits. The permeabilized cells were stained with the PerCP-Cy5.5 Mouse Anti-BrdU monoclonal antibody Cat. No. 560809 followed by the DNA-specific dye, DAPI dihydrochloride at 1 mug/mL Sigma, Cat. No. D9542 . Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII system.