BD HorizonTM APC-R700 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was stained with PE Mouse Anti-Human CD4 antibody 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody Cat. No. 565106/565107; Middle Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Plot - Human peripheral blood mononuclear cells were stimulated for 3 days with phytohemagglutinin. The cells were stained with either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon APC-R700 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.