BD HorizonTM APC-R700 Mouse Anti-Human IFN-gamma

Description

Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 561808/555333/561809 and either no antibody Autofluorescence Control; Left Panel or BD Horizon APC-R700 Mouse Anti-Human IFN-gamma antibody Cat No. 564981; Right Panel . Two-color flow cytometric contour plots showing the expression of IFN-gamma or cellular autofluorescence versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.