BD HorizonTM APC-R700 Mouse Anti-Human IL-17A

Description

Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-17A expression in stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PE Mouse Anti-Human CD4 antibody Cat. No. 555347/561843/561844 and either BD Horizon APC-R700 Mouse IgG1, kappa Isotype Control Cat. No. 564974; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-17A antibody Cat. No. 565163; Right Plot . Two-color flow cytometric dot plots show the correlated expression patterns of IL-17A or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.