BD HorizonTM APC-R700 Rat Anti-Human IL-10

Description

Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of IL-10 expressed in stimulated human lymphocytes. An enriched population of CD4+ peripheral blood mononuclear cells PBMC was obtained by panning using plate-bound Purified NA/LE Mouse Anti-Human CD4 antibody Cat. No. 555343 . The CD4+ PBMC were cultured 5 d with plate-bound NA/LE Mouse Anti-Human CD3 Cat. No. 555329; 10 mug/ml, coated overnight at 4C and soluble NA/LE Mouse Anti-Human CD28 Cat. No. 555725; 1 mug/ml antibodies plus recombinant Human IL-2 Cat. No. 554603; 20 ng/ml and IL-4 Cat. No. 554605; 40 ng/ml proteins. The cells were restimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA; Sigma P8139; 50 ng/ml and ionomycin Sigma I9657; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The cells were harvested, fixed using BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti- Human CD4 antibody Cat. No. 565997/566907 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; Left Plot or BD Horizon APC-R700 Mouse Anti-Human IL-10 antibody Cat. No. 567012/567013; Right Plot . A bivariate pseudocolor density plot showing the coexpressed levels of IL-10 or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.