BD HorizonTM APC-R700 Rat Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated mouse splenic leucocytes. Splenic leucocytes from a C57BL/6 mouse were cultured without Left Plot or with Right Plot plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 for 3 days. The resting Left Plot or activated Right Plot splenocytes were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , and stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histograms or BD Horizon APC-R700 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 566876; solid line histograms at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD276 PD-1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.