BD HorizonTM APC-R700 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD45 antibody Cat. No. 565478; solid line histogram . The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume. Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD45 antibody Cat. No. 565478; solid line histogram . The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume. Show More Flow cytometric analysis of CD45 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with either BD Horizon APC-R700 Rat IgG2b, kappa Isotype Control Cat. No. 564984; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD45 antibody Cat. No. 565478; solid line histogram . The fluorescence histogram showing CD45 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume.