BD HorizonTM APC-R700 Rat Anti-Mouse CD62L

Description

Flow cytometric analysis of mouse CD62L expression. Left Plots - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Cat. No. 565159; solid line histogram . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Right Plots - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Middle Right Plot or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume. Flow cytometric analysis of mouse CD62L expression. Left Plots - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Cat. No. 565159; solid line histogram . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Right Plots - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Middle Right Plot or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume. Show More Flow cytometric analysis of mouse CD62L expression. Left Plots - CD62L expression on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Plot or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Plot . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Cat. No. 564982; dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Cat. No. 565159; solid line histogram . The fluorescence histograms showing CD62L expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Right Plots - CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon APC-R700 Rat IgG2a, kappa Isotype Control Middle Right Plot or BD Horizon APC-R700 Rat Anti-Mouse CD62L antibody Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 mug per million cells in a 100 mul volume.