BD HorizonTM APC-R700 Streptavidin

Description

Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of mouse CD45R/B220 or human CD3 expression on leucocytes using Biotin-conjugated primary antibodies and BD Horizon APC-R700 Streptavidin. Left Plot - Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 555842; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; solid line histogram . The cells were washed and stained with BD Horizon APC-R700 Streptavidin Cat. No. 565144 . The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Right Plot - Human whole blood was stained with either Biotin Mouse IgG1 kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331; solid line histogram . Erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . The cells were washed and stained with BD Horizon APC-R700 Streptavidin. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.