BD HorizonTM BB515 Goat Anti-Mouse Ig

Description

Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes and CD4 expression on rat splenic leucocytes - Secondary staining comparisons between BD Horizon BB515- and FITC-conjugated Goat Anti-Mouse Ig. Left Panel: Human whole blood was either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG1, kappa Anti-Human CD3 antibody Cat. No. 555330; bold solid line histogram . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 followed by washing, and then staining the remaining leucocytes with BD Horizon BB515 Goat Anti-Mouse Ig Cat. No. 565060 . Alternatively, whole blood was stained with Purified Anti-Human CD3 antibody, treated with BD FACS Lysing Solution, followed by washing, and then secondary staining of the leucocytes with FITC Goat Anti-Mouse Ig Cat. No. 554001; thin solid line histogram . Right Panel: Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG2a, kappa Anti-Rat CD4 antibody Cat. No. 554835; bold solid line histogram followed by washing, and then staining with BD Horizon BB515 Goat Anti-Mouse Ig. Alternatively, the leucocytes were stained with Purified Anti-Rat CD4, washed, and then secondarily stained with FITC Goat Anti-Mouse Ig thin solid line histogram . Overlaid histograms are shown to facilitate secondary staining comparisons between BB515- or FITC-conjugated Goat Anti-Mouse Ig using purified mouse antibodies specific for human CD3 and rat CD4. The fluorescence histograms showing human CD3 or rat CD4 expression or control staining were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes or viable rat leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes and CD4 expression on rat splenic leucocytes - Secondary staining comparisons between BD Horizon BB515- and FITC-conjugated Goat Anti-Mouse Ig. Left Panel: Human whole blood was either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG1, kappa Anti-Human CD3 antibody Cat. No. 555330; bold solid line histogram . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 followed by washing, and then staining the remaining leucocytes with BD Horizon BB515 Goat Anti-Mouse Ig Cat. No. 565060 . Alternatively, whole blood was stained with Purified Anti-Human CD3 antibody, treated with BD FACS Lysing Solution, followed by washing, and then secondary staining of the leucocytes with FITC Goat Anti-Mouse Ig Cat. No. 554001; thin solid line histogram . Right Panel: Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG2a, kappa Anti-Rat CD4 antibody Cat. No. 554835; bold solid line histogram followed by washing, and then staining with BD Horizon BB515 Goat Anti-Mouse Ig. Alternatively, the leucocytes were stained with Purified Anti-Rat CD4, washed, and then secondarily stained with FITC Goat Anti-Mouse Ig thin solid line histogram . Overlaid histograms are shown to facilitate secondary staining comparisons between BB515- or FITC-conjugated Goat Anti-Mouse Ig using purified mouse antibodies specific for human CD3 and rat CD4. The fluorescence histograms showing human CD3 or rat CD4 expression or control staining were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes or viable rat leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes and CD4 expression on rat splenic leucocytes - Secondary staining comparisons between BD Horizon BB515- and FITC-conjugated Goat Anti-Mouse Ig. Left Panel: Human whole blood was either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG1, kappa Anti-Human CD3 antibody Cat. No. 555330; bold solid line histogram . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 followed by washing, and then staining the remaining leucocytes with BD Horizon BB515 Goat Anti-Mouse Ig Cat. No. 565060 . Alternatively, whole blood was stained with Purified Anti-Human CD3 antibody, treated with BD FACS Lysing Solution, followed by washing, and then secondary staining of the leucocytes with FITC Goat Anti-Mouse Ig Cat. No. 554001; thin solid line histogram . Right Panel: Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse Ms IgG2a, kappa Anti-Rat CD4 antibody Cat. No. 554835; bold solid line histogram followed by washing, and then staining with BD Horizon BB515 Goat Anti-Mouse Ig. Alternatively, the leucocytes were stained with Purified Anti-Rat CD4, washed, and then secondarily stained with FITC Goat Anti-Mouse Ig thin solid line histogram . Overlaid histograms are shown to facilitate secondary staining comparisons between BB515- or FITC-conjugated Goat Anti-Mouse Ig using purified mouse antibodies specific for human CD3 and rat CD4. The fluorescence histograms showing human CD3 or rat CD4 expression or control staining were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes or viable rat leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.