BD HorizonTM BB515 Mouse Anti-Human CD152

Description

Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD152 expression on activated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -activated 3 days; Stimulated human peripheral blood mononuclear cells were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . These cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 555335/561810/561811 and with either BD Horizon BB515 Mouse IgG2a, kappa Isotype Control Cat. No. 564515; Left Plot or BD Horizon BB515 Mouse Anti-Human CD152 antibody Cat. No. 566917/566918; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD152 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.