BD HorizonTM BB515 Mouse Anti-Human CD215 (IL-15Ralpha)

Description

Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes . Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without Left Plots or with Right Plots lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma protein Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219 , and then stained with APC Mouse Anti-Human CD14 antibofy Cat. No. 555399 and with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; Upper Plots or BD Horizon BB515 Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 567746/567747; Lower Plots . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL15Ralpha or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes . Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without Left Plots or with Right Plots lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma protein Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219 , and then stained with APC Mouse Anti-Human CD14 antibofy Cat. No. 555399 and with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; Upper Plots or BD Horizon BB515 Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 567746/567747; Lower Plots . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL15Ralpha or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD215 IL15Ralpha expression on human monocytes . Human peripheral blood mononuclear cells were cultured in complete tissue culture medium without Left Plots or with Right Plots lipopolysaccharide LPS, 100 ng/ml and Recombinant Human IFN-gamma protein Cat. No. 554617, 10 ng/ml for 20 hours at 37C. The cells were harvested, preincubated with Human BD Fc Block Cat. No. 564219 , and then stained with APC Mouse Anti-Human CD14 antibofy Cat. No. 555399 and with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; Upper Plots or BD Horizon BB515 Mouse Anti-Human CD215 IL15Ralpha antibody Cat. No. 567746/567747; Lower Plots . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of CD215 IL15Ralpha or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.