BD HorizonTM BB515 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left and Middle Panels: Human whole blood was stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 564467/564468; Middle Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus Autofluorescence were generated for events with the forward and side light- scatter characteristics of intact lymphocytes. Right Panel: Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin. The cells were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.