BD HorizonTM BB515 Mouse Anti-Human CD25

Description

Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 567318/567319; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 solid line histogram . A CD25 expression or Ig Isotype control staining histogram was derived from gated events with light-scatter characteristics of viable lymphoblasts. A BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used. Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 567318/567319; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 solid line histogram . A CD25 expression or Ig Isotype control staining histogram was derived from gated events with light-scatter characteristics of viable lymphoblasts. A BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used. Show More Two-color flow cytometric analysis of CD25 expression on unstimulated peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840/561841 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human CD25 antibody Cat. No. 567318/567319; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. CD25 expression on activated lymphocytes. Human PBMC were stimulated with PHA 3 days . Cells were stained with BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human CD25 solid line histogram . A CD25 expression or Ig Isotype control staining histogram was derived from gated events with light-scatter characteristics of viable lymphoblasts. A BD LSRFortessa X-20 Cell Analyzer System and FlowJo software were used.