BD HorizonTM BB515 Mouse Anti-Human CD39

Description

Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells - Staining comparisons between BD Horizon BB515- and FITC-conjugated CD39-specific antibodies. Human peripheral blood mononuclear cells were stained with BD Horizon PE-CF594 Mouse Anti-Human CD4 Cat. No. 562281/562316 , BD Horizon BV421 Mouse Anti-Human CD25 Cat. No. 562442/562443 , Alexa Fluor 647 Anti-Human CD127 Cat No. 558598/560905 antibodies, and costained with either BD Horizon BB515 Mouse IgG2b, kappa Isotype Control Cat. No. 564510; dashed line histogram , BD Horizon BB515 Mouse Anti-Human CD39 antibody Cat. No. 565469; bold solid line histogram , or FITC Mouse Anti-Human CD39 antibody Cat. No. 561444; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD39 antibody versus its Ig Isotype Control Middle Panel , and BB515 Anti-CD39 antibody versus FITC Anti-CD39 antibody Right Panel . The fluorescence histograms showing CD39 expression or Ig Isotype control staining were derived from CD4+CD25+CD127low-gated events ie, cells with a Regulatory T cell immunophenotype; Left Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.