BD HorizonTM BB515 Mouse Anti-Human CD40L (CD154)

Description

Flow cytometric analysis of CD40L CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dotted line histogram or BD Horizon BB515 Mouse Anti-Human CD40L CD154 antibody Cat. No. 568170/568171; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD40L CD154 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD40L CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dotted line histogram or BD Horizon BB515 Mouse Anti-Human CD40L CD154 antibody Cat. No. 568170/568171; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD40L CD154 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD40L CD154 expression on stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were stimulated for 4 hours with 20 ng/mL Phorbol 12-Myristate 13-Acetate PMA; Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL Calcium Ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dotted line histogram or BD Horizon BB515 Mouse Anti-Human CD40L CD154 antibody Cat. No. 568170/568171; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD40L CD154 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.