BD HorizonTM BB515 Mouse Anti-Human IgM

Description

Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes - Staining comparisons between BD Horizon BB515- and FITC-conjugated antibodies. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Panel , BD Horizon BB515 Mouse Anti-Human IgM antibody Cat. No. 564622; Middle Panel , or FITC Mouse Anti-Human IgM antibody Cat. No. 555782/562029; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.