BD HorizonTM BB515 Mouse Anti-Human IL-3Ralpha (CD123)

Description

Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plots or BD Horizon BB515 Mouse Anti-Human IL-3Ralpha CD123 Cat No. 567715/567716; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plots or BD Horizon BB515 Mouse Anti-Human IL-3Ralpha CD123 Cat No. 567715/567716; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Show More Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plots or BD Horizon BB515 Mouse Anti-Human IL-3Ralpha CD123 Cat No. 567715/567716; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes.