BD HorizonTM BB515 Mouse Anti-Human Integrin alpha1 (CD49a)

Description

Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 570076/570157; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 570076/570157; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human peripheral blood leukocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 570076/570157; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BB515 Mouse Anti-Human Integrin alpha1 CD49a antibody solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.