

566025 · 25 Tests
BDB Bioscience · Cat: 566025
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of NKp44 CD336 expression by IL-2-activated large granular lymphocytes. Human peripheral blood mononuclear cells were cultured 7 days, 37C with recombinant human IL-2 protein Cat. No. 554603 . The IL-2-activated cells were then stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies, and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dashed line histogram or BD Horizon BB515 Mouse Anti-Human NKp44 CD336 antibody Cat. No. 565099/566025; solid line histogram . The fluorescence histogram showing NKp44 CD336 expression or Ig Isotype control staining was derived from CD3- CD56+ gated events with the forward and side light-scatter characteristics of large granular lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of NKp44 CD336 expression by IL-2-activated large granular lymphocytes. Human peripheral blood mononuclear cells were cultured 7 days, 37C with recombinant human IL-2 protein Cat. No. 554603 . The IL-2-activated cells were then stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies, and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dashed line histogram or BD Horizon BB515 Mouse Anti-Human NKp44 CD336 antibody Cat. No. 565099/566025; solid line histogram . The fluorescence histogram showing NKp44 CD336 expression or Ig Isotype control staining was derived from CD3- CD56+ gated events with the forward and side light-scatter characteristics of large granular lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of NKp44 CD336 expression by IL-2-activated large granular lymphocytes. Human peripheral blood mononuclear cells were cultured 7 days, 37C with recombinant human IL-2 protein Cat. No. 554603 . The IL-2-activated cells were then stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 and APC Mouse Anti-Human CD56 Cat. No. 555518 antibodies, and either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; dashed line histogram or BD Horizon BB515 Mouse Anti-Human NKp44 CD336 antibody Cat. No. 565099/566025; solid line histogram . The fluorescence histogram showing NKp44 CD336 expression or Ig Isotype control staining was derived from CD3- CD56+ gated events with the forward and side light-scatter characteristics of large granular lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.