BD HorizonTM BB515 Mouse Anti-Human TACTILE (CD96)

Description

Two-color flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat No. 561811/555335/561810 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human TACTILE CD96 antibody Cat No. 568847/568848; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat No. 561811/555335/561810 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human TACTILE CD96 antibody Cat No. 568847/568848; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood lymphocytes. The flow cytometric data from the same stained cells was reanalyzed to generate the bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of TACTILE CD96 expression on Human peripheral blood leucocytes. Human whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat No. 561811/555335/561810 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Human TACTILE CD96 antibody Cat No. 568847/568848; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of TACTILE CD96 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.