BD HorizonTM BB515 Mouse Anti-Mouse CD199 (CCR9)

Description

Two-color flow cytometric analysis of CCR9 expression on mouse thymocytes and splenocytes. C57BL/6 mouse thymocytes Top Plots and splenic leucocytes Bottom Plots were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat No. 553035/561093 , and either BD Horizon BB515 Mouse IgG2a Isotype Control Cat. No. 564515; Left Plots or BD Horizon BB515 Mouse Anti-Mouse CD199 CCR9 Cat. No. 565577; Right Plots at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression of CD199 CCR9 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CCR9 expression on mouse thymocytes and splenocytes. C57BL/6 mouse thymocytes Top Plots and splenic leucocytes Bottom Plots were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat No. 553035/561093 , and either BD Horizon BB515 Mouse IgG2a Isotype Control Cat. No. 564515; Left Plots or BD Horizon BB515 Mouse Anti-Mouse CD199 CCR9 Cat. No. 565577; Right Plots at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression of CD199 CCR9 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CCR9 expression on mouse thymocytes and splenocytes. C57BL/6 mouse thymocytes Top Plots and splenic leucocytes Bottom Plots were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat No. 553035/561093 , and either BD Horizon BB515 Mouse IgG2a Isotype Control Cat. No. 564515; Left Plots or BD Horizon BB515 Mouse Anti-Mouse CD199 CCR9 Cat. No. 565577; Right Plots at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression of CD199 CCR9 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.