BD HorizonTM BB515 Mouse Anti-Mouse CD366 (TIM-3)

Description

Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD366 TIM-3 expressed on activated mouse splenocytes. BALB/c splenic leucocytes were cultured for 4 days in the presence of plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating , soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml , and Purified NA/LE Rat Anti-Mouse IL-4 Cat. No. 554432 antibodies. The cells were harvested, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416; Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD366 TIM-3 antibody Cat. No. 567810; Right Plot at 1 mug per test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD366 TIM-3 or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.