BD HorizonTM BB515 Mouse Anti-Mouse CD369 (CLEC7A)

Description

Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with APC Rat Anti-Mouse Ly-6G antibody Cat. No. 560599 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416 or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569836/569911; Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with APC Rat Anti-Mouse Ly-6G antibody Cat. No. 560599 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416 or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569836/569911; Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peritoneal exudate cells PEC . BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus F4/80 was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD369 CLEC7A expression on viable Mouse peripheral blood leukocytes PBL . BALB/c Mouse peripheral blood leukocytes PBL were stained with APC Rat Anti-Mouse Ly-6G antibody Cat. No. 560599 and with either BD Horizon BB515 Mouse IgG1, kappa Isotype Control Cat. No. 564416 or BD Horizon BB515 Mouse Anti-Mouse CD369 CLEC7A antibody Cat. No. 569836/569911; Right Plot at 0.5 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 CLEC7A or Ig Isotype control staining versus Ly-6G was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.