BD HorizonTM BB515 Rat Anti-Human CCR7 (CD197)

Description

Multicolor flow cytometric analysis of CCR7 CD197 expression on CD4+ and CD8+ human peripheral blood lymphocytes: staining comparisons between BD Horizon BB515- and FITC- conjugated antibodies. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , and either BD Horizon BB515 Rat Anti-Human CCR7 CD197 Cat. No. 565869/565870; Top Plots , or FITC Rat Anti-Human CCR7 CD197 Cat. No. 560548/561675; Bottom Plots antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CCR7 CD197 were derived from CD4+ Left Plots or CD8+ Right Plots gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of CCR7 CD197 expression on CD4+ and CD8+ human peripheral blood lymphocytes: staining comparisons between BD Horizon BB515- and FITC- conjugated antibodies. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , and either BD Horizon BB515 Rat Anti-Human CCR7 CD197 Cat. No. 565869/565870; Top Plots , or FITC Rat Anti-Human CCR7 CD197 Cat. No. 560548/561675; Bottom Plots antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CCR7 CD197 were derived from CD4+ Left Plots or CD8+ Right Plots gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of CCR7 CD197 expression on CD4+ and CD8+ human peripheral blood lymphocytes: staining comparisons between BD Horizon BB515- and FITC- conjugated antibodies. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 , BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 , and either BD Horizon BB515 Rat Anti-Human CCR7 CD197 Cat. No. 565869/565870; Top Plots , or FITC Rat Anti-Human CCR7 CD197 Cat. No. 560548/561675; Bottom Plots antibodies. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The two-color flow cytometric dot plots showing the correlated expression of CD45RA versus CCR7 CD197 were derived from CD4+ Left Plots or CD8+ Right Plots gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.