BD HorizonTM BB515 Rat Anti-Mouse CD209b (SIGN-R1)

Description

Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on viable BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 570048/570129; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or cellular Autofluorescence versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on viable BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 570048/570129; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or cellular Autofluorescence versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on viable BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with APC Rat Anti-CD11b antibody Cat. No. 553312 and with either no BD Horizon BB515 conjugated antibody Autofluorescence Control; Left Plot or BD Horizon BB515 Rat Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 570048/570129; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or cellular Autofluorescence versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable DAPI negative PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.