BD HorizonTM BB515 Rat Anti-Mouse CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Freshly prepared mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control Cat. No. 564417; Left Panel or BD Horizon BB515 Rat Anti-Mouse CD25 antibody Cat. No. 564424/564458; Middle Panel . Two-color flow cytometric contour plots showing the correlated expression patterns for Ig Isotype control staining Left Panel or CD25 expression Middle Panel versus cellular autofluorescence measured in the APC/allophycocyanin channel were generated for gated events with the forward and side light- scatter characteristics of viable lymphocytes. Right Panel: Mouse splenic leucocytes were stimulated with concanavalin A for 3 days. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with either BD Horizon BB515 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon BB515 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.